goat anti-momp primary antibody  (Meridian Bioscience)

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Lipopolysaccharide-Binding Alkylpolyamine DS-96 Inhibits Chlamydia trachomatis Infection by Blocking Attachment and Entry

doi: 10.1128/AAC.02391-14

Figure Lengend Snippet: DS-96 blocks attachment of C. trachomatis EB host cell surface. Two representative L929 cells that were incubated with EBs were pretreated with DS-96 (8 μM) or mock treated (DMSO) for an hour at 4°C. Infected cultures were thoroughly washed to remove unbound bacteria, fixed with methanol, and stained with fluorescently labeled anti-MOMP (Chlamydia; green), Evan's blue (cytosol; red), and DAPI (nuclei; blue).

Article Snippet: To stain the extracellular bacteria, the cells were incubated with primary goat anti-MOMP antibody (1621; ViroStat) diluted 1:500 in PBS for an hour at room temperature prior to permeabilization of host cells.

Techniques: Incubation, Infection, Bacteria, Staining, Labeling

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Lipopolysaccharide-Binding Alkylpolyamine DS-96 Inhibits Chlamydia trachomatis Infection by Blocking Attachment and Entry

doi: 10.1128/AAC.02391-14

Figure Lengend Snippet: Entry of C. trachomatis EBs into the host cell is inhibited by DS-96. C. trachomatis EBs were allowed to attach and enter host cells. Differential immunofluorescent staining was performed 4 hpi to discriminate between the extracellular and internalized EBs. (A) Representative images of infected cells. Host cell surface-associated EBs were labeled with anti-MOMP antibody (red) prior to cell permeabilization, and total EBs (intracellular plus extracellular) were labeled with anti-Chlamydia antibody (green) after permeabilization. The inset shows the internalized bacteria (green) in a mock-treated sample and the extracellular bacteria (yellow) in a DS-96-treated sample. (B) Percentage of EBs internalized relative to total EBs was quantified. The asterisk indicates that levels measured were significantly different (P < 0.001 by Student's t test) from those of DMSO-treated samples.

Article Snippet: To stain the extracellular bacteria, the cells were incubated with primary goat anti-MOMP antibody (1621; ViroStat) diluted 1:500 in PBS for an hour at room temperature prior to permeabilization of host cells.

Techniques: Staining, Infection, Labeling, Bacteria

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Lipopolysaccharide-Binding Alkylpolyamine DS-96 Inhibits Chlamydia trachomatis Infection by Blocking Attachment and Entry

doi: 10.1128/AAC.02391-14

Figure Lengend Snippet: Pretreatment of EBs with DS-96 results in reduction of anti-LOS staining. Purified EBs were treated with DS-96 (8 μM) or DMSO and stained for LOS (A) or MOMP (B) following methanol fixation. Samples were counterstained with DAPI to visualize the total number of bacteria.

Article Snippet: To stain the extracellular bacteria, the cells were incubated with primary goat anti-MOMP antibody (1621; ViroStat) diluted 1:500 in PBS for an hour at room temperature prior to permeabilization of host cells.

Techniques: Staining, Purification, Bacteria